Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme without the N-terminal signal peptide in Escherichia coli strain BL21(DE3) | Bifidobacterium longum subsp. Longum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibits 47% at 1 mM | Bifidobacterium longum subsp. Longum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for larch type II arabinogalactan is 7.86 mg/ml for the recombinant enzyme | Bifidobacterium longum subsp. Longum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates 24% at 1 mM | Bifidobacterium longum subsp. Longum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium longum subsp. Longum | A0A401ETL2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme without the N-terminal signal peptide from Escherichia coli strain BL21(DE3) | Bifidobacterium longum subsp. Longum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Bifidobacterium longum subsp. Longum | 4-nitrophenol + L-arabinofuranose | - |
? | |
arabinan + H2O | release of L-arabinofuranose | Bifidobacterium longum subsp. Longum | L-arabinofuranose + ? | - |
? | |
beta-D-Galp-(1->6)-[alpha-L-Araf-(1->3)]-beta-D-Galp-(1->6)-beta-D-Galp + H2O | recombinant BlArafA releases L-arabinose from the substrate, which is a beta-1,6-galactan side chain containing alpha-1,3-linked L-Araf | Bifidobacterium longum subsp. Longum | L-arabinofuranose + beta-D-Gal-(1->6)-beta-D-Gal-(1->6)-beta-D-Gal | - |
? | |
gum arabic + H2O | release of L-arabinofuranose, low activity | Bifidobacterium longum subsp. Longum | L-arabinofuranose + ? | - |
? | |
larch type II arabinogalactan + H2O | release of L-arabinofuranose, degradation profiles of type II AG | Bifidobacterium longum subsp. Longum | L-arabinofuranose + ? | - |
? | |
methyl alpha-L-Araf-(1->2)-alpha-L-Araf + H2O | low activity compared to methyl alpha-L-Araf-(1->3)-alpha-L-Araf | Bifidobacterium longum subsp. Longum | L-arabinofuranose + L-Araf-alpha-O-methyl ether | - |
? | |
methyl alpha-L-Araf-(1->3)-alpha-L-Araf + H2O | - |
Bifidobacterium longum subsp. Longum | L-arabinofuranose + L-Araf-alpha-O-methyl ether | - |
? | |
methyl alpha-L-Araf-(1->5)-alpha-L-Araf + H2O | low activity compared to methyl alpha-L-Araf-(1->3)-alpha-L-Araf | Bifidobacterium longum subsp. Longum | L-arabinofuranose + L-Araf-alpha-O-methyl ether | - |
? | |
additional information | the recombinant alpha-L-arabinofuranosidase releases arabinofuranose (Araf) from alpha-1,3-Araf-substituted beta-1,6-galactooligosaccharides. beta-1,6-Gal2 is additively released from larch type II arabinogalactan (AG) by the combined use of type II AG degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145). Degradative mechanism, overview. The enzyme shows 100, 86.1, and 69.1% specificities for radish root AGP, larch AG, and arabinan, respectively. Arabinoxylan is a poor substrate. BlArafA is an alpha-L-arabinofuranosidase, which recognizes oligosaccharides containing alpha-1,3-linked L-Araf structures in type II AG and arabinan | Bifidobacterium longum subsp. Longum | ? | - |
? | |
radish root arabinogalactan-protein + H2O | release of L-arabinofuranose | Bifidobacterium longum subsp. Longum | L-arabinofuranose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 133000, recombinant His-tagged enzyme without signal peptide, SDS-PAGE, x * 132981, His-tagged enzyme without signal peptide, sequence calculation | Bifidobacterium longum subsp. Longum |
Synonyms | Comment | Organism |
---|---|---|
BlAraf | - |
Bifidobacterium longum subsp. Longum |
blArafA | - |
Bifidobacterium longum subsp. Longum |
GH43_22 alpha-L-arabinofuranosidase | - |
Bifidobacterium longum subsp. Longum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Bifidobacterium longum subsp. Longum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
220 | - |
larch type II arabinogalactan | pH 5.5, 40°C, recombinant enzyme | Bifidobacterium longum subsp. Longum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant enzyme | Bifidobacterium longum subsp. Longum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 43, GH43, subfamily 5. GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) forms a gene cluster with GH30_5 and GH43_22 enzyme candidates, which are conserved in all Bifidobacterium longum strains | Bifidobacterium longum subsp. Longum |
metabolism | GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) | Bifidobacterium longum subsp. Longum |
physiological function | type II arabinogalactan (AG) degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are important for the acquisition of type II AG in Bifidobacterium longum | Bifidobacterium longum subsp. Longum |